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博士&硕士生导师

李福川

日期:2021-07-28点击:

李福川教授,博士生导师

山东省泰山产业领军人才

Tel: 0532-58631406

E-mail: fuchuanli@sdu.edu.cn

青岛市即墨区海滨大道72号山东大学青岛校区K6楼219室

学习经历

2000/09至2003/07,中国海洋大学,医药学院,博士研究生/博士

1997/09至2000/07,山东大学,生命科学院,硕士研究生/硕士

1992/09至1996/07,中南民族大学,化学系,本科/学士

研究经历

2011/06至今,山东大学,国家糖工程技术研究中心,教授,博士生导师,泰山产业领军人才

2007/02至2011/06,加拿大多伦多大学,分子细胞生物学部,博士后,导师Jorge Filmus教授

2006/04至2007/01,日本北海道大学,生命科学研究生院,博士后,导师Kazuyuki Sugahara教授

2003/10至2006/04,日本神户药科大学,生物化学系,日本学术振兴学会(JSPS)博士后,导师Kazuyuki Sugahara教授

研究方向

长期从事蛋白聚糖生物学研究,在世界上率先开展海洋微生物来源糖胺聚糖降解酶研究,并在糖胺聚糖及蛋白聚糖生物学,药用糖胺聚糖及类似物的分离纯化、结构鉴定和构效关系研究等方面有丰富研究经验,取得了一系列国际领先的研究成果。已在J. Cell Biol.,Chem. Sci.,J. Biol. Chem.,Ana. Chem.,Dev. Cell等相关国际著名期刊上发表系列蛋白聚糖生物学相关论文。

目前研究方向主要包括

  1. 生物来源新型多糖特别是糖胺聚糖降解酶的发现鉴定、催化机制、分子酶学改造与应用研究;

  2. 糖胺聚糖构效关系研究及相关医药产品的研发;

  3. 基于新型酶和探针的糖相关新型分析检测技术研发和应用;

  4. 细胞表面蛋白聚糖在胚胎发育和癌变过程中的细胞信号转导调控机制研究。

科研项目

曾承担和参与日本文部省、加拿大CIHR支持的各类科研项目十余项。近五年主持和承担包括863计划(2012AA021504,负责人)、973重大专项(2012CB822100,骨干)、国家自然科学基金(31570071,负责人)、山东省科技计划(2013GSF12106,2018GSF121002负责人)等多个国家及省部级课题。

代表性研究论文

1.Chune Peng , Qingbin Wang , Runmiao Jiao , Yingying Xu , Naihan Han, Wenshuang Wang, Changxiang Zhu,Fuchuan Li* A novel chondroitin sulfate E from Dosidicus gigas cartilage and its antitumor metastatic activityCarbohydr Polym.2021;262:117971

2.Qingdong Zhang,Hai-YanCao, LinWei,Danrong Lu, Min Du, MinYuan,Deling Shi, Xiangxue Chen, PengWang,Xiu-Lan Chen, Lianli Chi,Yu-zhongZhang*, andFuchuan Li*. Discovery ofexolytic heparinases and their catalytic mechanism and potential application.Nat. Commun.2021.12:1263

3.WenshuangWang,Cédric Przybylski, Xiaojuan Cai, Chrystel Lopin-Bon, Runmiao Jiao, Liran Shi, Kazuyuki Sugahara, José L. Neira, Régis Daniel*, andFuchuan Li*. Investigation of Action Pattern of a Novel Chondroitin Sulfate/Dermatan Sulfate 4-O-Endosulfatase.Biochem. J.2021. 478(2):281-298

4.WenshuangWang,Liran Shi,YongQin andFuchuan Li*. Research and application of chondroitin sulfate/dermatan sulfate-degrading enzymes.Front. CellDev.Biol.2020,10.3389/fcell.2020.560442.

5.WenshuangWang,Nanhan Han,YingyingXu,YunxueZhao, Liran Shi,Jorge Filmus, andFuchuan Li*. Assembling Custom Side Chains on Proteoglycans to Interrogate Their Function in Living Cells.Nat.Commun.2020,11:5915.

6.Qingdong Zhang, Danrong Lu, ShuminWang,LinWei,WenshuangWang,andFuchuan Li*.Identification and biochemical characterization of a novel chondroitin sulfate/dermantan sulfate lyase fromPhotobacteriumsp.Int. J. Biol. Macromol.2020, 165: 2314-2325.

7.Danrong Lu, Qingdong Zhang, ShuminWang,Jingwen Guan , Runmiao Jiao , Naihan Han,WenjunHan, andFuchuan Li*. Biochemical characteristics and synergistic effect of two novel alginate lyases fromPhotobacteriumsp. FC615.Biotechnol. Biofuels.2019, 12:260.

8.ShuminWang,Tiantian Su, Qingdong Zhang, Jingwen Guan, Jing He, Lichuan Gu, andFuchuanLi*. Comparative Study of Two Chondroitin Sulfate/Dermatan Sulfate 4-O-Sulfatases With High Identity.Front. Microbiol.2019, 10:1309.

9.ShuminWang,Jingwen Guan, Qingdong Zhang, Xiangxue Chen, andFuchuan Li*. Identificationand Signature Sequences of Bacterial Δ4,5Hexuronate-2-O-Sulfatases.Front. Microbiol.2019, 10: 704.

10.Chune Peng, QingbinWang,ShuminWang,WenshuangWang,Runmiao Jiao,WenjunHan, andFuchuan Li*. A chondroitin sulfate and hyaluronic acid lyase with poor activity to glucuronyl 4,6- O-disulfated N-acetylgalactosamine (E-type)-containing structures.J. Biol. Chem.2018, 293(12):4230-4243.

11.Chune Peng, QingbinWang,Danrong Lu,WenjunHan, andFuchuan Li*. A Novel BifunctionalEndolytic Alginate Lyase with Variable Alginate-Degrading Modes and Versatile Monosaccharide- Producing Properties.Front. Microbiol.2018, 9:167.

12.Naihan Han,WenshuangWang,Danrong Lu, HanWang,Xinying Ma, Xiaopeng Fan, andFuchuan Li*. A novel, rapid, and sensitive homogeneous sandwich detection method of Glypican-3 as a serum marker for hepatocellular carcinoma.Chem. Commun (Camb)., 2017, 53(90):12209-12212.

13.WenshuangWang,Xiaojuan Cai,Naihan Han,WenjunHan,Kazuyuki Sugahara,andFuchuan Li*. Sequencing of chondroitin sulfate oligosaccharides using a novel exolyase from a marine bacterium that degrades hyaluronan and chondroitin sulfate/dermatan sulfate.Biochem. J., 2017, 474(22):3831-3848.

14.WenshuangWang,JunhongWang,andFuchuan Li*.Hyaluronidase and Chondroitinase.Adv.Exp.Med. Biol., 2017, 925:75-87.

15.ShuminWang,Kazuyuki Sugahara, andFuchuan Li*.Chondroitin sulfate/dermatan sulfate sulfatases from mammals and bacteria.Glycoconj. J., 2016, 33(6):841-851.

16.Xiaoru Zhang, Ruijuan Li,YuanyuanChen, Shusheng Zhang,WenshuangWang,andFuchuan Li*. Applying DNA rolling circle amplification in fluorescence imaging of cell surface glycans labeled by a metabolic method.Chem.Sci.,2016,7,6182-6189.

17.WenjunHan,YuanyuanCheng, DandanWang,ShuminWang,Huihui Liu, Jingyan Gu, ZhihongWu, andFuchuan Li*. Biochemical Characteristics and Substrate Degradation Pattern of a Novel Exo-Type β-Agarase from the Polysaccharide-Degrading Marine BacteriumFlammeovirgasp. Strain MY04.Appl. Environ. Microbiol.2016, pii: AEM.00393-16.

18.WenjunHan, Jingyan Gu,YuanyuanCheng, Huihui Liu,YuezhongLi, andFuchuan Li*. NovelAlginate Lyase (Aly5) from a Polysaccharide-Degrading Marine Bacterium, Flammeovirga sp. Strain MY04: Effects of Module Truncation on Biochemical Characteristics, Alginate Degradation Patterns, and Oligosaccharide-Yielding Properties.Appl. Environ. Microbiol.2015, 82(1):364-74.

19.WenshuangWang,Naihan Han, Ruijuan Li,WenjunHan, Xiaoru Zhang, andFuchuan Li*. Supercharged Fluorescent Protein as aVersatileProbe for the Detection of Glycosaminoglycans inVitroand inVivo.Anal.Chem.2015, 87(18):9302-7.

20.WenshuangWang,WenjunHan, Xingya Cai,Xiaoyu Zheng, Kazuyuki Sugahara,andFuchuan Li*. Cloning and characterization of a novel chondroitin sulfate/dermatan sulfate 4-O-endosulfatase from a marine bacterium.J.Biol.Chem.2015, 290(12):7823-32.

21.WenjunHan,WenshuangWang,Mei Zhao, Kazuyuki Sugahara, andFuchuan Li*. A novel eliminase from a marine bacterium that degrades hyaluronan and chondroitin sulfate.J. Biol.Chem.2014, 289(40):27886-98.

22.Fuchuan Li,WenShi, Mariana I Capurro and Jorge Filmus. Glypican-5 stimulates rhabdomyosarcoma cell proliferation by activating hedgehog signaling.J. Cell Biol.,2011, 192(4):691-704.

23.Fuchuan Li, Chilkunda D. Nandini,TomohideHattori,Xingfeng Bao,Daisuke Murayama,Toshikazu Nakamura, Nobuhiro Fukushima,and Kazuyuki Sugahara.Structure of pleiotrophin-and hepatocyte growth factor-binding sulfated hexasaccharide determined by biochemical and computational approaches.J. Biol.Chem.,2010, 285(36):27673-85.

24.Mariana I Capurro,Fuchuan Liand Jorge Filmus. Overgrowth of a mouse model of Simpson- Golabi-Behmel syndrome is partly mediated by Indian hedgehog.EMBO Rep., 2009, 10(8):901-7.

25.Fuchuan Li,Gerdy B.ten Dam,Sengottuvelan Murugan, ShuheiYamada,TaishiHashiguchi,Shuji Mizumoto, Kayoko Oguri, Minoru Okayama,ToinH. van Kuppevelt, and Kazuyuki Sugahara. Involvement of Highly Sulfated Chondroitin Sulfate in the Metastasis of the Lewis Lung Carcinoma Cells.J. Biol.Chem.,2008, 283(49):34294-304.

26.Mariana I Capurro, Ping Xu,WenShi,Fuchuan Li, Angela Jia and Jorge Filmus. Glypican-3 Inhibits Hedgehog Signaling During Development by Competing with Patched for Hedgehog Binding.Dev.Cell,2008,14(5):700-11.

27.Fuchuan Li, Ajaya Kumar Shetty and Kazuyuki Sugahara. Neuritogenic activity ofchondroitin/dermatan sulfate hybrid chains of embryonic pig brain and their mimicry from shark liver. Involvement of the pleiotrophin and hepatocyte growth factor signaling pathways.J. Biol. Chem., 2007, 282(5): 2956-2966.

授权专利

1.一种难以降解CS-E的糖胺聚糖裂解酶及其编码基因与应用,李福川,彭春娥,王庆彬等, 国家发明专利,专利号:ZL201710544077.9

2.一种外切型糖胺聚糖裂解酶及其编码基因与应用,李福川,蔡晓娟,王文爽等,国家发明专利,专利号:ZL201610227396.2

3.一株弧菌FC509菌株及其培养方法与应用,李福川,韩文君,王文爽等,国家发明专利, 专利号:ZL201410159983.3

4.一种内切型硫酸软骨素硫酸皮肤素4-O-硫酸酯酶及其编码基因与应用,李福川,王文爽,郑小煜等,国家发明专利,专利号:ZL201410209975.5

5.一种糖胺聚糖降解酶及其编码基因与应用,李福川,韩文君,王文爽等,国家发明专利, 专利号:ZL2014101,60095.3

6.一种高表达水溶性肝素酶I融合蛋白及其编码基因, 李福川,赵梅,韩文君等,国家发明专利,专利号:ZL201410246947.0

7.一种外切型琼胶酶及其编码基因与应用,李福川,韩文君等,国家发明专利,专利号:

ZL201410396060.X

8.高正电荷荧光蛋白在糖胺聚糖分析检测中的应用,李福川,王文爽等,国家发明专利,专利号:ZL201510044900.0

9.一种用于蛋白聚糖高灵敏度检测的试剂盒及其制备方法,李福川,韩乃寒等,国家发明专利,专利号:ZL201610143751.8

10.一种内切型褐藻胶裂解酶及其编码基因与应用,李福川,韩文君等,国家发明专利,专利号:ZL2014104698614

11.一种高硫酸化硫酸软骨素及其制备方法与应用,李福川,王庆彬等,国家发明专利,专利号:ZL201610323540.2

12.一种外切型糖胺聚糖裂解酶及其编码基因与应用,李福川,王文爽等,国家发明专利,专利号:ZL2016102273962

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