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硕士生导师

孔蕴

日期:2021-07-28点击:



孔蕴

博士,副教授

电话:0532-58631411

E-mailkongyun@sdu.edu.cn

地址:山东省青岛市即墨滨海路72K6101


教育经历

2011/02-2014/10,哥本哈根大学,健康学院,细胞与遗传医学博士

2007/09-2010/07,山东大学,生命科学学院,微生物学硕士

2003/09-2007/07,山东大学,国家生命科学与技术人才培养基地,学士


研究经历

2020/09-今,山东大学,国家糖工程技术研究中心,副教授

2015/01-2020/9,山东大学,国家糖工程技术研究中心,助理研究员


研究方向

1. 结合基因编辑技术和高分辨率质谱的蛋白质糖基化修饰分析与功能学研究

2. 多糖与糖蛋白疫苗设计与生产研究;

3. 单克隆抗体等生物大分子药物的糖基化工程研究。


科研项目

1. 山东大学青年学者未来计划2020/09-2025/08,主持;

2. 山东省重点研发计划(公益类),利用 ENGase法在毕赤酵母中生产人源化糖蛋白药物,2019/01 -2020/12, 主持;

3. 国家自然科学基金面上项目,基于模块化组装策略快速制备细菌糖蛋白缀合物疫苗, 2021/01-2024/12, 参与人(第2位);

4. 横向项目重组新型冠状病毒(SARS-CoV-2SpikeS)蛋白的糖型分析,2022

5. 山东省重大科技创新工程,合成生物学工程化关键技术研发与应用,子课题,2020/12-2023/12, 参与人(第2位);

6. 国家自然科学基金青年基金项目O-甘露糖基转移酶底物特异性研究和黏蛋白型O-糖基化对其底物位点选择的影响, 2016/01-2018/12,主持;

7. 山东大学自主创新项目, 不同来源O-甘露糖基转移酶底物特异性研究,2015/7-2018/1,主持。


近期发表论文

【1】 Yongheng Rong# , Xingyun Wang, Weian Mao, Min Chen, Shengjun Wang , Peng George Wang*, Yunjiao He*, Yun Kong(孔蕴)*. O-GalNAc glycosylation affects the immunogenicity of the receptor-binding domain (RBD) of SARS-CoV-2 spike protein, Chem Commun (Camb), 2023 Feb 9;59(13):1797-1800.

【2】 Yongheng Rong#, Xingyun Wang, Weian Mao, Fang Yuan, Min Chen, Shengjun Wang, Peng George Wang, Zhigang Wu, Yunjiao He*, and Yun Kong(孔蕴)*. Chemoenzymatic Synthesis of SARS-CoV2 Homogeneous OLinked Glycopeptides for Exploring Their Inhibition Functions, ACS Infect. Dis, 2022, 8, 21982206.

【3】 Yun Kong(孔蕴)#, Fang Liu#, Zhaoxi Liu, Jiayu Zhao, Qizheng Wu, Xunlian Zhang, Min Liu, Houcheng Zhang, Songqiang Liu, Xiaomei Zhang*, Min Chen*. Synthesis of globotriose-modified peptides for the preparation of a colorimetric biosensor to detect Shiga toxins, Talanta, 2022, 243.

【4】 Li Kun#, Liu Zhaoxi, Liu Xiaoyu, Wang Lei, Zhao Jiayu, Zhang Xunlian, Kong Yun(孔蕴)*, Chen Min*. An anti-biofilm material: polysaccharides prevent the precipitation reaction of silver ions and chloride ions and lead to the synthesis of nano silver chloride, Nanotechnology, 2021, 32(31).

【5】 Shengjun Wang#, Yongheng Rong, Yaoguang Wang, Decai Kong, Peng George Wang, Min Chen, Yun Kong(孔蕴)*. Homogeneous production and characterization of recombinant N-GlcNAc-protein in Pichia pastoris, Microbial Cell Factories, 2020, 19(7).

【6】 Xunlian Zhang#, Weihao Sheng, Kun Li, Yongheng Rong, Qizheng Wu, Qingyun Meng, Yun Kong(孔蕴)*, Min Chen*. Substrate specificity of the galactokinase from the human gut symbiont Akkermansia muciniphila ATCC BAA-835, Enzyme and Microbial Technology, 139 (2020) 109568.

【7】 Qingzheng Wu#, Fang Liu, Yingjuan Song, Qingyun Meng, Xunlian Zhang, Huizi Li, Jingyu Sun, Hongqi Gao, Peng George Wang, Yun Kong(孔蕴)*, Min Chen*. A new strategy for high-throughput identification of human gut microbiota containing specific monosaccharide, Sci China Life Sci, 62(2019) 985-987.

【8】 Qingyun Meng#, Kun Li, Yongheng Rong, Qizheng Wu, Xunlian Zhang, Yun Kong(孔蕴)*, Min Chen*. Probing peptide substrate specificities of N-glycosyltranferase isoforms from different bacterial species, Carbohydrate Research, 473 (2019) 82–87.

【9】 Shengjun Wang#, Yaoguang Wang#, Peng George Wang, Min Chen, Yun Kong(孔蕴)*. High level expression and glycosylation of recombinant Mycobacterium tuberculosis Ala-Pro-rich antigen in Pichia pastoris, Protein Expression and Purification, 150 (2018) 67–71.

【10】 Yun Kong(孔蕴)#, Jiang Li, Xinyuan Hu, Yaoguang Wang, Qingyun Meng, Guofeng Gu, Peng George Wang, Min Chen*. N-Glycosyltransferase from Aggregatibacter aphrophilus synthesizes glycopeptides with relaxed nucleotide-activated sugar donor selectivity, Carbohydrate Research. 462 (2018) 7-12.

【11】 Yun Kong(孔蕴)#,Yajun Qu, Shengjun Wang , Peng George Wang, Min Chen*. Heterologous expression of Shigella dysenteriae serotype 1 O-antigen analog in Escherichia coli K-12 W3110 by transferring a minimum number of genes involved in O-polysaccharide biosynthesis, Biotechnology Letters, 40(2018) 1219–1226.

【12】 Yun Kong(孔蕴)#, Hiren J Joshi, Katrine Ter-Borch Gram Schjoldager, Thomas Daugbjerg Madsen, Thomas A Gerken, Malene B Vester-Christensen, Hans H Wandall, Eric Paul Bennett, Steven B Levery, Sergey Y Vakhrushev, Henrik Clausen*. Probing polypeptide GalNAc-transferase isoform substrate specificities by in vitro analysis, Glycobiology, 2015, 25(1): 55-65.

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