教育背景

博士2002.9-2005.7中国农科院生物技术所 生物化学及分子生物学

硕士1999.9-2002.7山东大学 微生物工程

工作经历

起止时间 单位名称 专业技术职务

2009.1-今 山东大学 副教授，教授

2005.9-2007.8 SCION（Ensis） 博士后

2007.8-2009.1奥克兰大学 博士后

研究方向

1.利用代谢工程改造微生物，用以生产高附加值产品或者化学品

2.研究和开发合成生物学的方法和工具

科研项目

1. 2011.1-2016.12用合成生物学方法构建生物基材料的合成新途径 科技部973项目

2. 2018.1-2021.12一种动态时序调控级联线路的构建及其对琥珀酸合成的调控机制研究 国家自然科学基金面上项目

3. 2011.1-2015.12 PDC-MhpF代谢通道构建及代谢流量优化机制的研究 国家自然科学基金面上项目

4. 2017.1-2019.12利用合成生物学策略高效合成5-氨基乙酰丙酸的关键技术研究 山东省科技发展计划

5. 2016.1-2018.12全阶段琥珀酸生产技术 山东省科技发展计划

6. 2010.1-2012.12厌氧生产琥珀酸的关键技术研究山东省科技发展计划

7. 2011-2014.12利用合成生物学手段时序调控ALA的合成 山东大学自主创新项目

代表性论文

1. He X, Chen Y, Liang Q*, Qi Q. Autoinduced AND Gate Controls Metabolic Pathway Dynamically in Response to Microbial Communities and Cell Physiological State. ACS Synth Biol. 2017, 17;6(3):463-470.

2. Li J, Li Y, Cui Z, Liang Q*, Qi Q. Enhancement of succinate yield by manipulating NADH/NAD+ ratio and ATP generation. Appl Microbiol Biotechnol. 2017 Apr;101(8):3153-3161.

3. Gao D, Luan Y, Liang Q*, Qi Q. Exploring the N-terminal role of a heterologous protein in secreting out ofEscherichia coli. Biotechnol Bioeng. 2016 Jun 4. doi: 10.1002/bit.26028.

4. Chang Y, Su T, Qi Q*, Liang Q*. Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system. Microb Cell Fact. 2016 Nov 15;15(1):195..

5. Zhang F, Li J, Liu H, Liang Q*, Qi Q. ATP-Based Ratio Regulation of Glucose and Xylose Improved Succinate Production. PLoS One. 2016 Jun 17;11(6):e0157775.

6. Gu P, Su T, Wang Q, Liang Q*, Qi Q. Tunable switch mediated shikimate biosynthesis in an engineered non-auxotrophic Escherichia coli. Sci Rep. 2016 Jul 13; 6:29745.

7. Liang, Q., F. Zhang, et al. (2015). "Comparison of individual component deletions in a glucose-specific phosphotransferase system revealed their different applications." Scientific Reports 5: 13200.

8. Liang Q, Qi Q. From a co-production design to an integrated single-cell biorefinery. Biotechnology Advances 32 (2014) 1328–1335.

9. Dongfang Gao, Yaqi Luan, Qian Wang, Quanfeng Liang* and Qingsheng Qi* Construction of cellulose utilizing Escherichia coli based on a secretable cellulose Gao et al. Microb Cell Fact (2015) 14:159

10. Zhao J, Wang Q, Li M, Heijstra B, Wang S,Liang Q*, Qi Q. Escherichia colitoxin genehipAaffects biofilm formation and DNA release. Microbiology (SGM). 2013, 159 : 632–639.

11. Li Y, Li M, Zhang X, Yang P,Liang Q*, Qi Q*. A novel whole-phase succinate fermentation strategy with high volumetric productivity in engineered scherichia coli. Bioresource Technology. 2013, 149: 333–340

12. Gu P, Kang J, Yang F, Wang Q,Liang Q*, Qi Q *. The improved L-tryptophan production in recombinant Escherichia coliby expressing the polyhydroxybutyrate synthesis pathway. Appl. Microb. Biotechnol. 2013, 97(9):4121-7.

13. Liang Q, Zhang H, Li S, Qi Q. Construction of stress-induced metabolic pathway from glucose to 1,3-propanediol inEscherichia coli. Appl. Microb. Biotech nol, 2011, 89:57-62

14. Wang Z, Gao C, Wang Q,Liang Q∗, Qi Q∗, Production of pyruvate in Saccharomyces cerevisiae through adaptive evolution and rational cofactor metabolic engineering. Biochemical Engineering Journal, 2012, 67 126–131

15. Liang Q, Wang Q, Gao C, Wang Z, Qi Q. The effect of cyclodextrins on the ethanol tolerance of microorganisms suggests potential application. J. Ind. Microbiol. Biotechnol. 2011. 38:753-756

16. Liu Z, Zhao J, Liang X, Li K, Xiao X, Zhu J, Sun Q,Liang Q*, Characterization of luminescentVibrio campbelliiLZ5 and its potential application in the detection of environmental heavy metals. 2012, Biotechnology and Applied Biochemistry, DOI: 10.1002/bab.1040

17. Liang Q, Lloyd-Jones G*.Sphingobium scionensissp. nov., a polycyclic aromatic hydrocarbon degrading bacterium isolated from contaminated soil. Int J Syst Evol Microbiol, 2010, 60:413-416

18. Liang Q*, Lloyd-Jones G. Formation of poly-β-hydroxybutyrate from polycyclic aromatic hydrocarbons bySphingobium scionensesp. WP01T. Proceedings - International Conference on Computer Distributed Control and Intelligent Environmental Monitoring, CDCIEM 2011, p 2448-2451.

19. Liang Q, Takeo M, Chen M, Zhang W, Xu Y, Lin M*. Genetic Analysis of a Chromosome-encoded Gene Cluster for the Metabolic Pathway that Converts Aniline to TCA cycle inDelftia tsuruhatensisAD9. Microbiology-SGM, 2005, 151:3435-3446.

20. Liang Q, Chen M, Xu Y, Zhang W, Ping S, Lu W, Song X, Wang W, Geng L Takeo M, Lin M*. Functional identification of gene cluster for the aniline metabolic pathway mediated by transposable element. Chinese Science Bulletin, 2005, 50:1612-1616.

21. Su T, Liu F, Gu P, Jin H, Chang Y, Wang Q, Liang Q, Qi Q*. A CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome. Sci Rep. 2016 Nov 24;6:37895. doi: 10.1038/srep37895.

22. Zhuang Q, Wang Q,Liang Qand Qi Q,. Synthesis of polyhydroxyalkanoates from glucose that contain medium-chain-length monomers via the reversed fatty acidβ-oxidation cycle inEscherichia coli. Metabolic Engineering (2014) 24:78–86.

23. Chunyan Luo, Zeling Zeng, Deming Gong, Cunyang Zhao, Quanfeng Liang, Cheng Zeng., Evaluation of monolaurin from camphor tree seeds for controlling food spoilage fungi.Food Control 46 (2014) 488e494

24. Gu P, Yang F, Li F,Liang Q, Qi Q (2013) Knocking out analysis of tryptophan permeases inEscherichia colifor improving L-tryptophan production. Appl Microbiol Biotechnol. 97:6677–6683

25. Li F, Wang Y, Gong K, Wang Q,Liang Q, Qi Q. Constitutive expression of RyhB regulates the heme biosynthesis pathway and increases the 5-aminolevulinic acid accumulation inEscherichia coli. 2013, DOI: 10.1111/1574-6968.12322

26. Li M, Wang J, Geng Y, Li Y, Wang Q,Liang Q, Qi Q *. A strategy of gene overexpression based on tandem repetitive promoters inEscherichia coli. Microbial Cell Factories. 2012, 11:19.

27. Wang Q, Zhuang Q,Liang Q, Qi Q*. Polyhydroxyalkanoic acids from structurally-unrelated carbon sources inEscherichia coli. Appl Microbiol Biotechnol, 2013, 97:3301–3307

28. Chen Q, Wang Q, Wei G,Liang Q, Qi Q*. Production inEscherichia coliof Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) with Differing Monomer Compositions from Unrelated Carbon Sources. Applied and Environmental Microbiology, 2011, 77:4886-4893

29. Kang Z, Du L, Kang J, Wang Y, Wang Q,Liang Q, Qi Q*. Production of succinate and polyhydroxyalkanoate from substrate mixture by metabolically engineeredEscherichia coli. Bioresource Technology. 2011, 102:6600-6604

30. Gao C, Wang Z,Liang Q, Qi Q*. Global transcription engineering of brewer's yeast enhances the fermentation performance under high-gravity conditions, Appl. Microb. Biotechnol, 2010, 87: 1821-1827 .

31. Heijstra BD, Pichler FB, Liang Q, Blaza RG and Turner SJ*. Extracellular DNA and Type IV pili mediate surface attachment by Acidovorax temperans. Antonie van Leeuwenhoek. 2009, 95:343-349

32. Geng L, Chen M,Liang Q, Takeo M, Lin M*. Functional analysis of a putative regulatory gene,tadR, involved in aniline degradation inDelftia tsuruhatensisAD9. Arch Microbiol. 2009, 191:603-614

33. Xin F, Wang S, L. Song Q,Liang Q, Qi Q*. Molecular identification and characterization of peptide: N-glycanase from Schizosaccharomyces pombe. Biochem. Bioph. Res. Co. 2008, 368: 907-912

BOOK

Industrial Biorefineries and White Biotechnology Publisher: Elsevier Science Ltd. Publication date: 1 August 2015. Publication City/Country: Oxford ISBN 10: 0444634533 ISBN 13: 9780444634535

所获专利

1.一种大肠杆菌工程菌株及其低氧发酵生产琥珀酸的应用201210349656.5

2.一种大肠杆菌工程菌株及其全阶段发酵生产琥珀酸的应用201310203945.9